The studies on membrane dynamics in urinary bladder umbrella cells were focused on proteins as important factors for maintaining the permeability barrier and their role as pathways modulators for the discoidal/fusiform endocytic vesicles which is one of the main features of the umbrella cells. However, our workgroup has dedicated to the study of lipid membrane composition and its biological impact in the urothelium. We first studied the lipid membrane composition of rat urothelium modified my dietary treatments differentiated in their fatty acid composition. Changes of lipid composition were related to the asymmetric unit membrane organization and permeability. Finally, we observed that the lipid composition was critically related to the intracellular pathways of discoidal/fusiform endocytic vesicles and their content. The purpose of this communication is to summarize the importance of lipids in the membrane organization and permeability of the apical plasma membrane and endocytic vesicles of umbrella cells.
[1]
Jost SP, Gosling JA, Dixon JS (1989) The morphology of normalhuman bladder urothelium. J Anat 167:103–115.
[2]
Kreft ME, Hudoklin S, Jezernik K, Romih R (2010) Formation and maintenance of blood-urine barrier in urothelium. Protoplasma 246(1–4):3–14.
[3]
Grasso EJ and Calderón RO (2013) Membrane lipids and proteins as modulators of urothelial endocytic vesicles pathways. Histochem Cell Biol 140(5):507-520.
[4]
Hicks RM (1975) The mammalian urinary bladder: an accommodating organ. Biol Rev Camb Philos Soc 50(2):215–246
[5]
Wu XR, Manabe M, Yu J, Sun TT (1990) Large scale purification and immunolocalization of bovine uroplakins I, II, and III. Molecular markers of urothelial differentiation. J Biol Chem 265(31):19170–19179
[6]
Yu J, Manabe M, Wu XR, Xu C, Surya B, Sun TT (1990) Uroplakin I: a 27-kD protein associated with the asymmetric unit membrane of mammalian urothelium. J Cell Biol 111(3):1207–1216
[7]
Calderon RO, Glocker M, Eynard AR (1998) Lipid and fatty acid composition of different fractions from rat urinary transitional epithelium. Lipids 33(10):1017–1022
[8]
Wu XR, Lin JH, Walz T, Häner M, Yu J, Aebi U, Sun TT (1994) Mammalian uroplakins. A group of highly conserved urothelial differentiation-related membrane proteins. J Biol Chem 269(18):13716–13724
[9]
Liang F, Kachar B, Ding M, Zhai Z, Wu XR, Sun TT (1999) Urothelial hinge as a highly specialized membrane: detergent-insolubility, urohingin association, and in vitro formation. Differentiation 65(1):59-69.
[10]
Chang A, Hammond TG, Sun TT, Zeidel ML (1994) Permeability properties of the mammalian bladder apical membrane. Am J Physiol 267(5 Pt 1):C1483–C1492
[11]
Calderón RO, Eynard AR (2000) Fatty acids specifically related to the anisotropic properties of plasma membrane from rat urothelium. Biochim Biophys Acta 1483(1):174–184
[12]
Bongiovanni GA, Eynard AR, Caldero´n RO (2005) Altered lipid profile and changes in uroplakin properties of rat urothelial plasma membrane with diets of different lipid composition. Mol Cell Biochem 271(1–2):69–75
[13]
Ketterer B, Hicks RM, Christodoulides L, Beale D (1973) Studies of the chemistry of the luminal plasma membrane of rat bladder epithelial cells. Biochim Biophys Acta 311(2):180–190
[14]
Walz T, Häner M, Wu XR, Henn C, Engel A, Sun TT, Aebi U (1995) Towards the molecular architecture of the asymmetric unit membrane of the mammalian urinary bladder epithelium: a closed ‘‘twisted ribbon’’ structure. J Mol Biol 248(5):887–900
[15]
Min G, Stolz M, Zhou G, Liang F, Sebbel P, Stoffler D, Glockshuber R, Sun TT, Aebi U, Kong XP (2002) Localization of uroplakin Ia, the urothelial receptor for bacterial adhesin FimH, on the six inner domains of the 16 nm urothelial plaque particle. J Mol Biol 317(5):697–706
[16]
Calderon RO, Grasso EJ (2006) Symmetric array of the urothelium surface controlled by the lipid lattice composition. Biochem Biophys Res Commun 339(2):642–646
[17]
Marsh D (1999) Thermodynamic analysis of chain-melting transition temperatures for monounsaturated phospholipid membranes: dependence on cis-monoenoic double bond position. Biophys J 77(2):953–963
[18]
Funari SS, Barceló F, Escribá PV (2003) Effects of oleic acid and its congeners, elaidic and stearic acids, on the structural properties of phosphatidylethanolamine membranes. J Lipid Res 44(3):567–575
[19]
de Kruijff B (1997) Biomembranes. Lipids beyond the bilayer. Nature 386:1676–1681
[20]
Hainau B (1979) Luminal plasma membrane organization in rat urinary bladder urothelium after direct exposure in vivo to N-methyl-N-nitrosourea. Cancer Res 39(9):3757–3762
[21]
Hu P, Deng FM, Liang FX, Hu CM, Auerbach AB, Shapiro E, Wu XR, Kachar B, Sun TT (2000) Ablation of uroplakin III gene results in small urothelial plaques, urothelial leakage, and vesicoureteral reflux. J Cell Biol 151(5):961–972
[22]
Hu P, Meyers S, Liang FX, Deng FM, Kachar B, Zeidel ML, Sun TT (2002) Role of membrane proteins in permeability barrier function: uroplakin ablation elevates urothelial permeability. Am J Physiol Renal Physiol 283(6):F1200–F1207
[23]
Grasso EJ, Calderón RO (2009) Urinary bladder membrane permeability differentially induced by membrane lipid composition. Mol Cell Biochem 330(1–2):163–169
[24]
Grasso EJ, Bongiovanni GA, Pérez RD, Calderón RO (2011) Precancerous changes in urothelial endocytic vesicle leakage, fatty acid composition, and as and associated element concentrations after arsenic exposure. Toxicology 284(1–3):26–33
[25]
Wimley WC, Selsted ME, White SH (1994) Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores. Protein Sci 3(9):1362–1373
[26]
Ladokhin AS, Wimley WC, White SH (1995) Leakage of membrane vesicle contents: determination of mechanism using fluorescence requenching. Biophys J 69(5):1964–1971
[27]
Seddon JM (1990) Structure of the inverted hexagonal (HII) phase, and non-lamellar phase transitions of lipids. Biochim Biophys Acta 1031(1):1–69
[28]
Wasungu L, Stuart MC, Scarzello M, Engberts JB, Hoekstra D (2006) Lipoplexes formed from sugar-based gemini surfactants undergo a lamellar-to-micellar phase transition at acidic pH. Evidence for a non-inverted membrane-destabilizing hexagonal phase of lipoplexes. Biochim Biophys Acta 1758(10):1677–1684
