Various immunosuppressive drugs have been mainly used for treatment of inflammatory bowel disease (IBD); however, their side effects and toxicity remain a major clinical concern. As a result, there is an increasing interest in using herbal medicine as an alternative and adjunct treatment in addition to the conventional therapies. As a suitable rabbit colitis model is currently not available, we developed a rabbit colitis model by administration of dextran sodium sulphate (DSS). Extraction of Dill Oil (DO) and Fennel Oil (FO). White Himalayan rabbits were acclimatized for 7days and then separated into five groups each of five rabbits. Group (1) (-ve) control, Group (2) DSS (+ve) control, Group(3) DSS+DO (1ml/day,5%), Group (4)DSS+FO (1ml/day,3.5%) and Group (5) DSS+DO+FO (0.5ml each per day). At the end of the experiment, rabbits will be sacrificed and the entire colon will be excised. Total glutathione will be determined in the colon homogente as well as, total RNA will be isolated from the colon homogenate, quantitative real-time PCR (qRT-PCR) reactions will be performed. Quantification of gene expression, Rabbit-specific primers will be designed for the genes of interest. The housekeeping gene β-actin will be used as an internal control, and quantification of the transcripts will be performed by the {Delta}{Delta}CT method. Data will be expressed as mean± standard error of means (SEM). Comparisons will be performed by one-way ANOVA. Expected outcomes Upon detection of new treatments will be the entrance to produce a potent therapeutic preparation can be manufactured locally and marketed after making stages rely followed.
[1]
Fiocchi C. Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology 1998;115:182–205.
[2]
Dyson JK, Rutter MD. Colorectal cancer in inflammatory bowel disease: what is the real magnitude of the risk? World J Gastroenterol 2012;18:3839–48.
[3]
Papadakis KA, Targan SR. Role of cytokines in the pathogenesis of inflammatory bowel disease. Annu Rev Med 2000;51:289–98.
[4]
Stokkers PC, Hommes DW. New cytokine therapeutics for inflammatory bowel dis- ease. Cytokine 2004;28:167–73.
[5]
Andresen L, Jørgensen VL, Perner A, Hansen A, Eugen-Olsen J, Rask-Madsen J. Activation of nuclear factor kappaB in colonic mucosa from patients with collagenous and ulcerative colitis. Gut 2005;54:503–9.
[6]
Singh K, Chaturvedi R, Barry DP, Coburn LA, Asim M, Lewis ND, et al. The apolipoprotein E-mimetic peptide COG112 inhibits NF-kappa B signaling, proinflammatory cytokine expression, and disease activity in murine models of colitis. J Biol Chem 2011;286:3839–50.
[7]
[7] Lawrance IC, Wu F, Leite AZ, Willis J, West GA, Fiocchi C, et al. A murine model of chronic inflammation-induced intestinal fibrosis down-regulated by antisense NF- kappa B. Gastroenterology 2003;125:1750–61.
[8]
Li Z, Zhang deK, Yi WQ, Ouyang Q, Chen YQ, Gan HT. NF-kappaB p65 antisense oli gonucleotides may serve as a novel molecular approach for the treatment of patients with ulcerative colitis. Arch Med Res 2008;39:729–34.
[9]
Nielsen OH, Bjerrum JT, Herfarth H, Rogler G. Recent advances using immunomodulators for inflammatory bowel disease. J Clin Pharmacol 2013;53:575–88.
[10]
Poitras P, Gougeon A, Binn M, Bouin M. Extra digestive manifestations of irritable bowel syndrome: intolerance to drugs? Dig Dis Sci 2008;53:2168–76.
[11]
Park MY, Ji GE, Sung MK. Dietary kaempferol suppresses inflammation of dextran sulfate sodium-induced colitis in mice. Dig Dis Sci 2012;57:355–63.
[12]
Callan, N.W., D.L. Johnson, M.P. Westcott and L.E. Welty, 2007. Herb and oil composition of dill (Anethum graveolens L.): Effects of crop maturity and plant density. Industrial Crops and Prod., 25: 282-287.
[13]
Bailer, J., T. Aichinger, G. Hackl, K. Hueber and M. Dachler, 2001. Essential oil content and composition in commercially available dill cultivars in comparison to caraway. Industrial Crops and Products., 14: 229-239.
[14]
Singh, G., S. Maurya, M.P. De Lampasona and C. Catalan, 2005. Chemical constituents, antimicrobial investigations and antioxidative potentials of Anethum graveolens L. Essential Oil and Acetone Extract: Part 52. J. Food Sci., 70: M208-M215.
[15]
Renjie, L., L. Zhenhong and S. Shidi, 2010. GC-MS analysis of fennel essential oil and its effect on microbiology growth in rat intestine. Afr. J. Microbiol. Res., 4: 1319-1323.
[16]
Shahat, A.A., A.Y. Ibrahim, S.F. Hendawy, E.A. Omer, F.M. Hammouda, F.H. Abdel-Rahman and M.A. Saleh, 2011. Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules, 16: 1366-1377.
[17]
Ozbek, H., S. Ugras, I. Bayram, I. Uygan, E. Erdogan, A. Ozturk and Z. Huyut, 2004. Hepatoprotective effect of Foeniculum vulgare essential oil: A carbon-tetrachloride induced liver fibrosis model in rats. Scand. J. Lab. Anim. Sci., 1: 9-17.
[18]
Miguel, M.G., C. Cruz, L. Faleiro, M.T. Simoes, A.C. Figueiredo, J.G. Barroso and L.G. Pedro, 2010. Foeniculum vulgare sential oils: chemical composition, antioxidant and antimicrobial activities. Nat. Prod. Commun., 5: 319-28.
[19]
Choi, E.M. and J.K. Hwang, 2004. Anti-inflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia, 75: 557-565.
[20]
Asher, M. Histological findings in rabbits which died with symptoms of mucoid enteritis. Laboratory Animals (1976) 10, 199-202. 199.
[21]
Rehg JE, and Pakes SP. Implication of Clostridium difficile and Clostridium perfringens iota toxins in experimental lincomycin-associated colitis of rabbits. Lab Anim Sci. 1982 Jun;32(3):253-7.
[22]
Keel, M. K. and Songer, J. G. The Comparative Pathology of Clostridium difficile–associated Disease, Review article. Vet Pathol 43:225–240 (2006).
[23]
European Commission (2000). Possible links between Crohn’s disease and Paratuberculosis. Report of the Scientific Committee on Animal Health and Animal Welfare. Adopted 21 March 2000.
[24]
The center for food security & Public Health. Enterohemorrhagic Escherichia coli Infections. Last Updated: May 2009.The center for food security & public health, Iowa State University, College of Veterinary Medicine.
[25]
Matthew, B. and Alan, F. (2011). Animal Models of Colitis: Lessons Learned, and Their Relevance to the Clinic, Ulcerative Colitis - Treatments, Special Populations and the Future, Dr Mortimer O'Connor (Ed.), ISBN: 978-953-307-739-0, InTech, Available from: http://www.intechopen.com/books/ulcerative-colitistreatments-special-populations-and-the-future/animal-models-of-colitis-lessons-learned-and-their-relevanceto- the-clinic.
[26]
Mee, a. S., Mclaughlin j. E., Hodgson, h. J. and Jewell, d. P. Chronic immune colitis in rabbits. Gut 1979: 20, 1-5.
[27]
Vineeta Khare, Kyle Dammann, Mario Asboth, DI, Anita Krnjic, Manuela Jambrich, and Christoph Gasche Overexpression of PAK1 Promotes Cell Survival in Inflammatory. Bowel Diseases and Colitis-associated Cancer. Inflamm Bowel Dis 2015;21:287–296.
[28]
Fabio Cominelli, Cynthia C. Nast, Rafael Uerena, Charles A. Dinarello and Robert D. Zipser. Interleukin I Suppresses Inflammation in Rabbit Colitis Mediation by Endogenous Prostaglandins. J. Clin. Invest. 1990. 85:582-586.
[29]
Rabeh, N. M., Aboraya A.O. Hepatoprotective Effect of Dill (Anethum graveolens L.) and Fennel (Foeniculum vulgare) Oil on Hepatotoxic Rats. Pakistan Journal of Nutrition 2014:13 (6): 303-309.
[30]
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2_DDCt method. Methods (2001) 25:402–408.
